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A Gut Reaction: Hoofprint Biome’s Innovative Approach to Slashing Cattle Methane Emissions

Cows on the alpine meadow
Courtesy: Unsplash

In a significant advancement for sustainable agriculture, Hoofprint Biome has announced the closing of a robust $15 million Series A funding round. Led by SOSV and supported by heavyweights including Amazon’s Climate Pledge Fund, Breakthrough Energy Fellows, and Alexandria Venture Investments, this investment marks a pivotal moment in the pursuit of more environmentally friendly livestock farming. The round also enjoyed participation from all existing investors such as AgriZeroNZ, Twynam, Good Growth Capital, and Ponderosa Ventures.


Unlike traditional approaches that have targeted methane emissions through chemicals and drugs, Hoofprint Biome is pioneering a groundbreaking method that utilizes the natural processes within the cattle’s own digestive system. “For too long, our strategies have been focused externally. What if the answer has been inside the cattle all along, in the rumen itself?” Po Bronson, General Partner at SOSV, proposed during the announcement.


Scott Collins, the Chief Scientific Officer at Hoofprint Biome, explains that their technology employs biotechnology and artificial intelligence to harness proteins evolved by nature to regulate microbial growth in the gut. “These proteins are then optimized to effectively reshape the microbiome, reducing methane emissions substantially,” Collins adds.


This revolutionary enzyme-based feed additive not only promises to cut methane production but also enhances feed efficiency. Kathryn Polkoff, CEO of Hoofprint Biome, highlights the dual benefits: “Our enzymes not only curb methane emissions but also redirect the energy flows in the rumen to boost milk production in dairy cows and increase weight in beef cattle, without compromising animal health or productivity.”


The company is now gearing up to translate these innovations from the lab to the field with partnerships for on-farm trials lined up as the next step. Nick Ellis, Principal at Amazon’s Climate Pledge Fund, emphasizes the importance of such innovations: “The dual challenges of reducing emissions and improving animal health are crucial. This technology could redefine standards in our beef and dairy industries.”


Produced through a scalable, cost-effective fermentation process, these enzymes integrate seamlessly into existing livestock nutrition practices, potentially facilitating broad adoption. Wayne McNee, Chief Executive of AgriZeroNZ, supports this optimism: “These enzymes and probiotics represent powerful tools for our farmers, enabling them to not only meet but exceed market demands for sustainable products.”


Cattle, vital for converting inedible plant material into high-quality proteins on lands unsuitable for cultivation, are also a significant source of methane—a potent greenhouse gas. By tackling this issue head-on, Hoofprint Biome is not just innovating; they are leading the charge towards a sustainable future in agriculture.


Launched in 2023 by a team of experts including Scott Collins and Kathryn Polkoff, Hoofprint Biome is driving a new era of efficient, climate-smart cattle farming from its base in Raleigh, North Carolina.

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